2 5x sybr one step rt pcr buffer (Sangon Biotech)

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Sangon Biotech 2 5x sybr one step rt pcr buffer
2 5x Sybr One Step Rt Pcr Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rt+pcr+buffer/buffer+pcr/pmc12753487-41-28-38
Average 86 stars, based on 1 article reviews

2 5x sybr one step rt pcr buffer - by Bioz Stars, 2026-10

86/100 stars

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Article Title: Ultra-sensitive fluorescence-activated droplet single-cell sorting based on Tetramer-HCR-EvaGreen amplification.
Article Snippet: Phosphate buffer solution (PBS), TM buffer, tris (hydroxymethyl) aminomethane (Tris), TE buffer, TBE buffer, and magnesium chloride (MgCl2) were purchased from Sangon Biotechnology Inc. One-Step RT-qPCR Kit and total RNA Extractor (Trizol) were purchased from Sangon Biotechnology Inc. Optiprep was purchased from SigmaAldrich Company Ltd.

Article Title: LncRNA NEAT1 participates in diminished ovarian reserve by affecting granulosa cell apoptosis and estradiol synthesis via the miR-204-5p/ESR1 axis
Article Snippet: KGN cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.5% Triton X-100 for 10 min. Pre-hybridization buffer (containing 50% formamide and 2× SSC buffer) was added to the cells that were incubated for 30 min, followed by overnight incubation in a hybridization buffer containing a fluorescein isothiocyanate (FITC)-labeled NEAT1 probe (Sangon Biotech, Shanghai, China).

Article Title: Ultra-sensitive fluorescence-activated droplet single-cell sorting based on Tetramer-HCR-EvaGreen amplification
Article Snippet: Phosphate buffer solution (PBS), TM buffer, tris (hydroxymethyl) aminomethane (Tris), TE buffer, TBE buffer, and magnesium chloride (MgCl 2 ) were purchased from Sangon Biotechnology Inc. One-Step RT-qPCR Kit and total RNA Extractor (Trizol) were purchased from Sangon Biotechnology Inc. Optiprep was purchased from Sigma-Aldrich Company Ltd.

Knockdown:

Article Title: LINC00184 promotes esophageal squamous cell carcinoma progression via DNMT1-mediated methylation of the NDRG2 promoter and PI3K/AKT pathway activation
Article Snippet: .. The detection of knockdown efficiency by RT-qPCR adopted the identical reagent system, thermal cycling parameters and calculation method as aforementioned in the RT-qPCR section. siRNA #2, which produced the highest knock-down (>70% reduction) was selected for all subsequent loss-of-function assays. siRNA duplexes specifically targeting mouse DNMT1 transcript: Sense (S), 5′-GCUGGGAGAUGGCGUCAUA-3′; antisense (AS), 5′-CAGGGAGAUACCGCAGUAU-3′; or a random non-coding mRNA sequence: S, 5′-UUCUCCGAACGUGUCACGUTT-3′; AS, 5′-ACGUGACACGUUCGGAGAATT-3′ were synthesized and reconstituted in 1X siRNA buffer (Sangon Biotech Co., Ltd.). ..

Quantitative RT-PCR:

Article Title: Manipulation of host-plant preference by virus-induced changes to its insect vector’s olfactory system
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-GST Abmart Cat#M20007; RRID: AB_2864360 Mouse monoclonal anti-MBP Proteintech Cat#66003-1-IG; RRID: AB_11183040 Mouse monoclonal anti-Flag Abmart Cat#M20008; RRID: AB_2713960 Mouse monoclonal anti-HA Abmart Cat#M20003; RRID: AB_2864345 Rabbit monoclonal anti-DIF This paper N/A Mouse monoclonal anti-GAPDH Proteintech Cat#60004-1-IG; RRID: AB_2107436 Rabbit polyclonal anti-Histone H3 Abmart Cat#P30266; RRID: AB_2936509 Rabbit monoclonal anti-Pelle This paper N/A Bacterial and virus strains Southern rice black-streaked dwarf virus Key Laboratory of Pesticide and Chemical Biology N/A Y2HGold yeast strain Weidi Biology Cat#YC1002 Biological samples Sogatella furcifera Key Laboratory of Pesticide and Chemical Biology N/A Oryza sativa L. Key Laboratory of Pesticide and Chemical Biology N/A Chemicals, peptides, and recombinant proteins E-β-farnesene Macklin Cat#18794-84-8 β-carophyllene Sigma-Aldrich Cat#87-44-5 Hexane Sigma-Aldrich Cat#110-54-3 Linalool Sigma-Aldrich Cat#78-70-6 2-heptanone Sigma-Aldrich Cat#110-43-0 α-pinene Sigma-Aldrich Cat#80-56-8 Methyl salicylate Sigma-Aldrich Cat#119-36-8 E-linalool oxide Sigma-Aldrich Cat#126-91-0 Sesquisabinene Hairui Cat#58319-05-4 3-hexen-1-ol Sigma-Aldrich Cat#928-96-1 α-copaene Sigma-Aldrich Cat#80-56-8 Critical commercial assays Eastep Super Total RNA Isolation Kit Promega Cat#LS1040 M-MLV Reverse Transcriptase Accurate Biology Cat#AG11605 2X SYBR Green SupTaq HS Premix Accurate Biology Cat#AG11762 T7 RiboMAXTM Express RNAi System Promega Cat#P1700 RNeasy MinElute Cleanup Kit Qiagen Cat#74204 CytoBusterTM Protein Extraction Reagent Merck Millipore Cat#71009-M Protein A + G Agarose Beyotime Technology Cat#P2012 One Step Insect Tissue Active Protein Extraction Kit Sangon Biotech Cat#C510020-0020 pIMAGO®-biotin Phosphoprotein Detection Kit TYMORA Cat#800-40 Alexa Fluor 488-labeled oligonucleotides Sangon Biotech Cat#B548614 FuGENE HD Transfection Reagent Promega Cat#E2311 Dual-Luciferase Reporter Assay Kit Promega Cat#E1910 Lambda Protein Phosphatase New England Biolabs Cat#P0753S glutathione Sepharose 4B beads Beyotime Technology Cat#P2251 (Continued on next page) ll OPEN ACCESS Current Biology 35, 1–14.e1–e6, August 4, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER amylose resin New England Biolabs Cat#E8022S trypan blue Beyotime Technology Cat#C0069S 5% Normal goat serum Sangon Biotech CatE510009 Fetal Bovine Serum Gibco Cat#10091-148 Tenax TA 60/80 Merck Millipore Cat#11982 Experimental models: Cell lines Sf9 cell line Key Laboratory of Pesticide and Chemical Biology N/A Experimental models: Organisms/strains Drosophila melanogaster: Or22ab-GAL4 Gift from Prof. Feng Liu (Shenzhen Bay Laboratory) N/A D. melanogaster Balancer: Sp/Cyo; Dr/TM3 Gift from Prof. Feng Liu (Shenzhen Bay Laboratory) N/A D. melanogaster: Or22ab-GAL4; UAS-SfOr86 This paper N/A D. melanogaster: Or22ab-GAL4; UAS-SfOr127 This paper N/A D. melanogaster: UAS-SfOr86 This paper N/A D. melanogaster: UAS-SfOr127 This paper N/A Oligonucleotides Primers for qRT-PCR are listed in Table S1 Sangon Biotech N/A dsRNA sequence used in this study are listed in Table S1 Sangon Biotech N/A Recombinant DNA dsDNA probe: Or86 This paper N/A dsDNA probe: Or127 This paper N/A dsDNA probe: Mutant Or86 This paper N/A dsDNA probe: Mutant Or127 This paper N/A Plasmid: pGBK Key Laboratory of Pesticide and Chemical Biology N/A Plasmid: pGAD Key Laboratory of Pesticide and Chemical Biology N/A Plasmid: pUAST.attb Gift from Prof. Feng Liu (Shenzhen Bay Laboratory) N/A Plasmid: pGL3-basic Miaoling Cat#P0193 Plasmid: pRL-CV40 Miaoling Cat#P48500 Software and algorithms Graphpad prism 8 GraphPad Software http://www.premierbiosoft.com/ primerdesign/ IBM SPSS Statistics 26 International Business Machines Corporation https://spss.en.softonic.com/ iTOL web server Interactive tree of life https://itol.embl.de/ MEGA-X MEGA https://www.megasoftware.net/ EAG Syntech http://www.ockenfel-syntech.com/ products/signal-acquisition-systems-2/ Autospike v.3.10 Syntech http://www.ockenfel-syntech.com/ products/signal-acquisition-systems-2/ GC-EAD Syntech http://www.ockenfel-syntech.com/ products/signal-acquisition-systems-2/ ll OPEN ACCESS e2 Current Biology 35, 1–14.e1–e6, August 4, 2025 Article .. S. furcifera from a laboratory-reared stock colony (Key Laboratory of Pesticide and Chemical Biology, South China Agricultural University, Guangzhou, China) were maintained at 27 ◦C with 70% relative humidity and a 16:8 h (L:D) photoperiod.

Article Title: Engineering a CRISPR-Mediated Dual Signal Amplification-Based Biosensor for miRNA Determination
Article Snippet: Tris (2-carboxyethy) phosphine hydrochloride (TCEP), 6-mercaptohexanol (MCH), bovine serum albumin (BSA), Tris(hydroxymethyl)aminomethane (Tris), and hydroquinone (HQ) were obtained from Aladdin Chemistry Co., Ltd. (Shanghai, China). .. The microRNA extraction kit, one-step RT-qPCR Kit, agarose B, dNTP (10 mM), diethylpyrocarbonate-treated water (DEPC-DW), 4S Gelred, 1 × TE buffer (10 mM Tris-HCl, 1.0 mM EDTA, pH 8.0), phosphate buffer saline (PBS), and 0.4 mg/mL SA-HRP were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). .. Nuclease-free water, Nb.BbvCI (Nb, 10 U/μL), Klenow Fragment (3′→5′ exo-, KF, 5 U/μL), EnGen Lba Cas12a (Cpf1) nuclease (100 μM), terminal deoxynucleotidyl transferase (TdT, 20 U/μL), NEBuffer 1, 10 × TdT buffer, and CoCl 2 solution (2.5 mM) were provided by New England Biolabs Inc. (Beijing, China); 2 × SYBR green mix, RNase Inhibitor (40 U/μL), and Annealing buffer (AB) were acquired from Beyotime Biotech Co., Ltd. (Shanghai, China).

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Article Title: C6‑HSL enhances BPA tolerance in Pseudomonas asiatica : an integrative Raman‑DIP and transcriptomic study
Article Snippet: Deuterium oxide (D 2 O, D113906), BPA (B108653) and C6-HSL (H275885) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. Ethyl acetate (34858), methanol (1.06035) and acetonitrile (1.00029) were purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd. .. The bacterial RNA extraction kit used was TRIzolTM MaxTM Bacterial RNA Isolation Kit (16096-020, Life Technologies, USA), and the PCR kits, Abstart One Step RT-PCR Mix (B110025) and 2.5X SYBR One Step RT-PCR Buffer (B110032), were purchased from Sangon Biotech (Shanghai) Co., Ltd. ..

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